TOXICOLOGICAL EFFECT OF PLANT FLAVONOIDS ON MCF-7,A-549 AND PA-1 CANCER CELL LINES

Abstract

The research aimed in the direction of five different medicinal plants (hydro alcoholics extract) to evaluate anticancer activity and cytotoxcity. The most important purpose of this study is to evaluate cytotoxicity of these medicinal plants with facilitate of MTT assay. Concentrations are prepared of each plant extracts which are 100 μg/ml, 10 μg/ml, 0.1 μg/ml, 0.01 μg/ml and 5-10×10 3 cells/ml are taken into each well which are exposed to different Concentrations of plant extracts crude and elute of column chromatography [CC] for 96 hrs and then treated with MTT and take absorbance at 570 nm. Standard drug was used for IC50 of Doxorubicin MCF-7 500nm, A549- 550nm, PA-1- 580nm.